Study of copper metabolism and liver damage in TX Mice-an animal model for liver disease.
- Author:
Xi CHEN
1
;
Chu-huai WANG
;
Yan-qing FENG
;
Qi-qiang TANG
;
Qiu-you XIE
;
Qi LIANG
;
Xiu-ling LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aspartate Aminotransferases; blood; Brain; metabolism; Ceruloplasmin; metabolism; Copper; metabolism; Disease Models, Animal; Female; Kidney; metabolism; Liver; metabolism; pathology; Liver Diseases; blood; metabolism; pathology; Male; Mice; Mice, Inbred Strains; Time Factors
- From: Chinese Journal of Hepatology 2009;17(9):688-690
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide right time points in selection of right aged animals and the normal physiological data of TX mice.
METHODS7-12 months old TX and DL mice were studied, each group contained 3 female and 3 male mice of TX or DL mice. The concentration of copper in the serum, dry tissues (liver, brain and kidney), together with copper biochemistry indexes were measured. The liver histopathology was observed under light microscopy and electron microscope.
RESULTSTransaminase increased significantly only in 10 and 11-month- old (AST(TX10) = 218.3 U/L, AST(TX11) = 197.5 U/L, AST(DL10) = 171.5 U/L, AST(DL11) = 165.0 U/L, P(10) less than 0.001, P(11) = 0.022), but the copper concentration of liver, brain and kidney was significantly increased during 7-12 month old (the average concentration of copper, Liver(TX) = (750.0 +/- 85.5) mg/kg, Brain(TX) = (39.7 +/- 2.2)mg/kg, Kidney(TX) = (29.8 +/- 5.0) mg/kg, Liver(DL) = (11.6 +/- 1.5) mg/kg, Brain(DL) = (16.8 +/- 0.9) mg/kg, Kidney(DL) = (14.2 +/- 1.0) mg/kg, t = 21.16, 23.60, 7.47, for all these organs P less than 0.05).
CONCLUSIONTX mice is a suitable model of liver disease with natural recovery, so selecting animal model of suitable time point is very important.
