Alteration of Expression of Ca(2+) Signaling Proteins and Adaptation of Ca(2+) Signaling in SERCA2(+/-) Mouse Parotid Acini.
10.3349/ymj.2008.49.2.311
- Author:
Jong Hoon CHOI
1
;
Hae JO
;
Jeong Hee HONG
;
Syng Ill LEE
;
Dong Min SHIN
Author Information
1. Department of Oral Medicine, Oral Science Research Center, Center for Natural Defense System, BK21 Project, Yonsei University College of Dentistry, Seoul, Korea. dmshin@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
sarco/endoplasmic reticulum Ca(2+)-ATPase;
Ca(2+) signaling proteins, parotid gland acinar cells;
parotid gland acinar cells
- MeSH:
Amylases/metabolism;
Animals;
Blotting, Western;
Calcium/metabolism;
Calcium Signaling/drug effects/genetics/*physiology;
Carbachol/pharmacology;
Immunohistochemistry;
Inositol 1,4,5-Trisphosphate Receptors/metabolism;
Mice;
Mice, Knockout;
Parotid Gland/*metabolism;
Sarcoplasmic Reticulum Calcium-Transporting ATPases/genetics/*metabolism;
Signal Transduction/drug effects/genetics/physiology
- From:Yonsei Medical Journal
2008;49(2):311-321
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca(2+) signaling. However, whether the changes in Ca(2+) signaling and Ca(2+) signaling proteins in parotid acinar cells are affected by a partial loss of SERCA2 are not known. MATERIALS AND METHODS: In SERCA2(+/-) mouse parotid gland acinar cells, Ca(2+) signaling, expression levels of Ca(2+) signaling proteins, and amylase secretion were investigated. RESULTS: SERCA2(+/-) mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca(2+) ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP(3)Rs), but the localization and activities of IP3Rs were not altered. In SERCA2(+/-) mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagmin was increased compared to wild type mice. CONCLUSION: These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca(2+) signaling proteins in the parotid gland acini, however, overall Ca(2+) signaling is unchanged.
