Identification of metastasis-related microRNAs of hepatocellular carcinoma in hepatocellular carcinoma cell lines by quantitative real time PCR.
- Author:
Yue ZHAO
1
;
Hu-liang JIA
;
Hai-jun ZHOU
;
Qiong-zhu DONG
;
Li-yun FU
;
Zhao-wei YAN
;
Jian SUN
;
Ning REN
;
Qing-hai YE
;
Lun-xiu QIN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Line; Cell Line, Tumor; DNA, Complementary; genetics; Epithelial Cells; metabolism; Humans; Liver Neoplasms; genetics; metabolism; pathology; MicroRNAs; genetics; metabolism; Neoplasm Metastasis; Polymerase Chain Reaction
- From: Chinese Journal of Hepatology 2009;17(7):526-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the metastasis-related miRNAs in hepatocellular carcinoma (HCC) cell lines.
METHODSA qRT-PCR method was established and optimized.
RESULTSAll candidate metastasis associated miRNAs except miR-124a were expressed in high metastasis cell line MHCC97H and low metastasis cell line MHCC97L, while some miRNAs were differentially expressed between liver cancer cell line (HepG2) and hepatic cell line (L02) (P less than 0.05), these miRNAs include: miR-148b (1.96+/-0.51 vs 3.76+/-0.28), miR-9 (-4.38+/-0.86 vs -1.10+/-0.53), miR-30c (8.41+/-0.40 vs 6.82+/-0.29), miR-338 (3.14+/-0.29 vs -2.36+/-0.32), miR-34a (0.71+/-0.40 vs -2.95+/-0.26), Let-7g (-4.07+/-0.55 vs -6.98+/-0.56). miR-148b expression was about 4 times higher than miR-148a [5.46 (IQR 4.25-6.67) vs 1.29 (IQR 0.94-1.64)] in all cell line tested (Z=-5.097, P=3x10(-7)).
CONCLUSIONThis study may help to understand the biological significance of miRNAs in HCC metastasis.
