Hepatitis B virus X protein regulates c-met promoter via the ERKs singal pathway in HepG2 cells.
- Author:
Bin XIE
1
;
Chun TANG
;
Ping CHEN
;
Yuan-bin GOU
;
Tao YUAN
;
Shi-long JIN
;
Yu-yang ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Butadienes; pharmacology; Extracellular Signal-Regulated MAP Kinases; antagonists & inhibitors; Gene Expression Regulation, Neoplastic; Genetic Vectors; Hep G2 Cells; Hepatitis B virus; genetics; Humans; Neoplasm Invasiveness; Neoplasm Metastasis; Nitriles; pharmacology; Plasmids; genetics; Promoter Regions, Genetic; genetics; Proto-Oncogene Proteins c-met; metabolism; Signal Transduction; Trans-Activators; genetics; metabolism; Transfection
- From: Chinese Journal of Hepatology 2009;17(7):531-534
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the signal pathway mediating the regulatory effect of Hepatitis B virus X protein (HBX) on c-met gene promoter in HepG2 cells.
METHODSThe expression of c-met in HBX-transfected HepG2 cells treated with different signal pathway inhibitors was detected by western blot, the invasion capability of cells was determined by Matrigel invasion assay.
RESULTSERK inhibitor U0126 inhibited the expression of the c-Met in HBx-transfected HepG2 cells. However, both p38MAPK inhibitor SB203580 and PI-3K inhibitor wortmanin had no effect on expression of the c-Met in HBx-transfected HepG2 cells. Furthermore, the ERK inhibitor U0126 also inhibited the invasiveness of HBX-transfected HepG2 cells.
CONCLUSIONHBx induces invasion of HCC via activation of ERK pathway.
