Induction of hepatic specification of human adipose-derived stem cells (hADSCs) in vitro.
- Author:
Min WANG
1
;
Hai-yun PEI
;
Li-dong GUAN
;
Xue NAN
;
Ci-xian BAI
;
Hui LIU
;
Bao-wei LI
;
Yun-fang WANG
;
Xue-tao PEI
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; cytology; Albumins; metabolism; Cell Culture Techniques; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cell Separation; Cells, Cultured; Culture Media; Fibroblast Growth Factor 2; pharmacology; Hepatocyte Growth Factor; pharmacology; Hepatocytes; cytology; metabolism; Humans; Mesenchymal Stromal Cells; cytology; Reverse Transcriptase Polymerase Chain Reaction; alpha-Fetoproteins; metabolism
- From: Chinese Journal of Hepatology 2009;17(7):544-548
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo induce hepatic differentiation of human adipose-derived stem cells (hADSCs) in vitro.
METHODShADSCs were isolated from human adipose tissue and treated with improved hepatic medium containing HGF, bFGF and FGF4. After 7 days of culture, OSM was added to the culture media. Cell growth during hepatic differentiation was evaluated by CCK8 assay. Morphology of differentiation was examined under light microscope. Liver specific genes and proteins were detected by RT-PCR analysis and immunohistochemical staining, respectively. And functional characteristics of hepatocytes were also examined.
RESULTSThe number of hADSCs cultured in the improved hepatic media was increased significantly in comparison to hADSCs cultured in control media from 5 days to 21 days (t=6.59, 8.69, 15.94 and 24.64, respectively, P<0.05). The hADSCs-derived hepatocyte-like cells exhibited hepatocyte morphology, expressed hepatocyte markers, possessed hepatocyte-specific activities, such as uptake and excretion of indocyanine green, glycogen storage and albumin production.
CONCLUSIONhADSCs can be induced into hepatocyte-like cells in this differentiation system. And this differentiation system promoted the growth of hADSCs.
