Curcumine inhibits migration and invasion of hepatic stellate cells by reducing MMP-2 expression and activity.
- Author:
Jian-xian HUANG
1
;
Bao-he ZHU
;
De HE
;
Lin HUANG
;
Ke HU
;
Bo HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cell Movement; drug effects; Cells, Cultured; Concanavalin A; administration & dosage; pharmacology; Curcumin; administration & dosage; pharmacology; Dose-Response Relationship, Drug; Hepatic Stellate Cells; cytology; drug effects; metabolism; Liver Cirrhosis; prevention & control; Matrix Metalloproteinase 2; metabolism; Rats; Tissue Inhibitor of Metalloproteinase-2; administration & dosage; pharmacology
- From: Chinese Journal of Hepatology 2009;17(11):835-838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular mechanism of the inhibitory effect of curcumine on the migration and invasion of hepatic stellate cells (HSC).
METHODSRat hepatic stellate cells were cultured and activated with ConA. Matrix metalloproteinase-2 (MMP-2) expression and activity was determined by Western blot and gelatin zymography. Migration and invasion of HSC was assessed by wound healing assay and modified Boyden chamber assay.
RESULTSCurcumine reduced the level and activity of MMP-2 expression in activated HSC in a dose-dependent manner. When treated with 25, 50 or 100 micromol/L curcumine, the expression of MMP-2 was reduced by 21.8%+/-5.1%, 65.5%+/-9.2% or 87.9%+/-11.5% (P < 0.05), and the activity of MMP-2 was also significantly reduced by curcumine. Migration and invasion of activated HSC was also inhibited by curcumine in a dose-dependent way. When treated with 25, 50 or 100 micromol/L curcumine, the migration of activated HSC was reduced by 27.5%+/-5.8%, 54.4%+/-7.6% or 67.1%+/-9.3% (P < 0.05), and the invasion of activated HSC was also significantly reduced by curcumine.
CONCLUSIONCurcumine inhibits migration and invasion of activated HSC by reducing MMP-2 expression and activity.
