Study of the bone-like apatite's depositing induced by collagen I with its mechanism.
- Author:
Jianglei TU
1
;
Fuqiang GUO
;
Chunchun LU
;
Bogang LI
Author Information
1. College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apatites;
metabolism;
Collagen Type I;
pharmacology;
Osteogenesis;
drug effects;
Skin;
chemistry;
Swine
- From:
Journal of Biomedical Engineering
2011;28(1):99-103
- CountryChina
- Language:Chinese
-
Abstract:
The collagen I was made with the dialysis method of enzymolysising the pig skin, and the static deposition in vitro of calcium phosphate was comparative studied by X-ray diffraction (XRD) and infrared spectroscopy (FTIR) under the condition of pH7. 4, Ca/P 1.67 and whether adding the collagen I into the system. Then the chemical composition of the sedimentary product and the diversification of the collagen I 's IR and Raman spectra (RS) before and after the mineralization were analyzed. The results showed that,under the physiological pH condition that there was not any collagen I, though Ca/P reached up to 1.67, the sedimentary product was CaHPO4 x 2H2O yet, however, after adding collagen I into the system, the bone-like apatite was deposited, which proved that collagen I indeed had the effects on the inducing of the bone-like apatite's mineralization in vitro; there was obviously mutual coordination action between collagen I and its mineralization product--bone-like apatite, which caused that amide peak I red-shifted, amide peak II and amide peak III decreased significantly or disappeared on the IR of collagen I, which maybe was the mechanism that how collagen I induced the depositing of the bone-like apatite.
