Controlled release of fuctional proteins IGF-1, aFGF and VEGF through self-assembling peptide nanofiber hydrogel.
- Author:
Yanfei LIU
1
;
Min WU
;
Bo LIN
;
Xiaojun ZHAO
Author Information
1. Institute of Nanobiomnedical Technology and Membrane, Sichuan University, Chengdu 610041, China. l_yfei@163.com
- Publication Type:Journal Article
- MeSH:
Circular Dichroism;
Delayed-Action Preparations;
Fibroblast Growth Factor 1;
metabolism;
Hydrogels;
chemistry;
Insulin-Like Growth Factor I;
metabolism;
Nanofibers;
Peptides;
chemistry;
Protein Structure, Secondary;
Vascular Endothelial Growth Factor A;
metabolism
- From:
Journal of Biomedical Engineering
2011;28(2):310-313
- CountryChina
- Language:Chinese
-
Abstract:
Peptide RADA can undergo spontaneous assembly into well ordered nanofibers and hydrogels in its water solution. In this work, a variety of proteins, including IGF-1, aFGF and VEGF with different molecular weight and isoelectric points, were chosen and encapsulated within the RADA peptide hydrogel. UV-vis spectroscopy was used to determine the concentration of the released proteins in the solution. The release kinetics suggested that protein diffusion through nanofiber hydrogels depended primarily on the size of the protein and the density of the peptide nanofiber. Circular dichroism (CD) spectroscopy indicated that the encapsulation and release by RADA hydrogel did not affect the secondary structure of the proteins studied.
