PLA-O-CMC nanoparticles: HGF loading and delivery behaviors in vitro.
- Author:
Zhifeng LI
1
;
Zhong CHEN
;
Ren'an CHANG
Author Information
1. Department of Surgery, Affiliated Maternal and Child Care Service Centre of Nantong University, Nantong 226001, China.
- Publication Type:Journal Article
- MeSH:
Chitosan;
analogs & derivatives;
chemistry;
Drug Carriers;
chemistry;
Hepatocyte Growth Factor;
metabolism;
Lactic Acid;
chemistry;
Nanoparticles;
chemistry;
Oxygen;
chemistry;
Polyesters;
Polymers;
chemistry
- From:
Journal of Biomedical Engineering
2011;28(2):314-317
- CountryChina
- Language:Chinese
-
Abstract:
This paper is aimed to observe the hepatocyte growth factor (HGF) loading and delivery ability of polylactic acid and oxygen carboxymethylated chitosan copolyer nanoparticles (PLA-O-CMC NPs). We prepared PLA-O-CMC NPs loaded with HGF by ultrasound in combination with magnetic stirring method. The NPs were characterized by transmission electron microscopy, embedding ratio; drug loading and drug delivery behaviors were observed by ELISA. The characteristics of PLA-O-CMC NPs loaded with HGF showed that the mean size was 139. 82 nm, polydispersity was 0.108, maximal HGF-embedding ratio was 76. 32%. The cumulative HGF release gradually increased in the first 24 hours in vitro, with sharp increasing in the first 7 hours, and moderate and steady increasing in the following 17 hours. The HGF had a burst release in the first 24 hours, and in this process the released HGF took up 36.7% of the whole release. From the second day,the HGF release decreased obviously, while it kept on releasing steadily (45-55 ng/d) for quite long time up to 30 days. The experiment proved that PLA-O-CMC NPs is a favourable carrier of HGF. PLA-O-CMC NPs loaded with HGF could rapidly release HGF in vitro. The released HGF reached the effective drug concentration and maintained the certain effective drug concentration for a long time.
