The expression of X-linked inhibitor of apoptosis protein and cell apoptosis in caerulein-stimulated rat pancreatic acinus AR42J cell lines.
- Author:
Jingjing JIANG
1
;
Zongguang ZHOU
;
Ling WANG
;
Lihui CHEN
;
Yuan LI
;
Hui YAN
;
Bin ZHOU
;
Yong LIU
;
Keling CHEN
Author Information
1. Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Acinar Cells;
cytology;
metabolism;
Animals;
Apoptosis;
physiology;
Cell Line;
Ceruletide;
pharmacology;
NF-kappa B;
metabolism;
Pancreas;
cytology;
metabolism;
Pancreatitis;
metabolism;
Rats;
X-Linked Inhibitor of Apoptosis Protein;
genetics;
metabolism
- From:
Journal of Biomedical Engineering
2011;28(2):332-351
- CountryChina
- Language:Chinese
-
Abstract:
To study the expression of X-linked inhibitor of apoptosis protein (XIAP) and cell apoptosis in vitro model of acute pancreatitis (AP), we carried out experiments to stimulate AR42J cell line with caerulein (10(-8) mol/L) for 12 hours, then collected cells at various time points (0 h, 4 h, 8 h, 12 h, and 24 h, respectively). We then observed the morphologic changes of AR42J cells with the stimulation of caerulein with electronic microscope. The gene expression of XIAP, caspase-3 and caspase-9 was detected using real-time fluorescence quantitative polymerase chain reaction (FQ-PCR), and the protein expression of XIAP was assessed by western blot. The activation of nuclear factor-kappa B (NF-kappaB) was measured by flow cytometry (FCM). With the stimulation of caerulein, the expression of XIAP and the NF-kappaB activation could first decrease and then increase, but the change of caspase-3 and caspase-9 expressions were opposite. XIAP may inhibit the cell apoptosis in rat pancreatic acinus AR42J cell lines at first with the stimulation of caerulein, then NF-kappaB can upgrade the expression of XIAP and increase the cell apoptosis.
