Expressions of COX-2, PKC-α and miR-101 in gastric cancer and their correlations.
- Author:
Haibing SUN
1
;
Yongchang WEI
;
Honglei TU
;
Ning DU
;
Yang ZHAO
;
Lijuan HU
;
Hong REN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Cyclooxygenase 2; metabolism; Female; Gastric Mucosa; metabolism; Humans; Male; MicroRNAs; metabolism; Middle Aged; Neoplasm Staging; Protein Kinase C-alpha; metabolism; Stomach Neoplasms; metabolism; pathology
- From: Journal of Southern Medical University 2013;33(4):559-562
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of miR-101, protein kinase C-α (PKC-α), and cyclooxygenase-2 (COX-2) in gastric cancer (GC) tissue and their correlations.
METHODSRT-qPCR was used to examine miR-101 expression and Western blotting employed to detect PKC-α and COX-2 expressions in 57 cases of gastric cancer tissues and paired normal gastric mucosal tissues.
RESULTSThe gastric cancer tissues showed a significantly lower miR-101 expression (Z=6.102, P<0.05) but significantly higher expressions of COX-2 (Z=14.436, P<0.05) and PKC-α (Z=6.955, P<0.05) than the normal gastric tissues. The expression of COX-2 protein was significantly correlated with the degree of differentiation, invasion depth, lymph node metastasis and TNM stage (P<0.05); PKC-α protein expression was associated with lymph node metastasis and TNM stage (P<0.05). PKC-α expression was positively correlated (r=0.531, P<0.05) and miR-101 expression negatively correlated (r=-0.627, P<0.05) with COX-2 expression in gastric cancer tissues.
CONCLUSIONSmiR-101, PKC-α and COX-2 all play a role in the tumorigenesis and progression of gastric cancer. miR-101 and PKC-α might be new potential therapeutic targets for inhibiting COX-2 in gastric cancer.
