Expression pattern of hsa-miR-9 and its association with BCL6 in EBV-positive and EBV-negative Burkitt lymphoma cell lines.
- Author:
Xinzhen DAI
1
;
Shaohong CHEN
;
Juan GE
;
Xiqun HAN
;
Xinhua ZHOU
;
Ziqin WU
;
Tong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Burkitt Lymphoma; genetics; pathology; virology; Cell Division; Cell Line, Tumor; DNA-Binding Proteins; genetics; metabolism; Gene Expression Regulation, Neoplastic; Herpesvirus 4, Human; Humans; MicroRNAs; metabolism; Proto-Oncogene Proteins c-bcl-6; Transfection
- From: Journal of Southern Medical University 2013;33(5):661-666
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differential expression pattern of hsa-miR-9 between EBV-positive and -negative Burkitt lymphoma cell lines and its association with BCL-6.
METHODSThe expression of hsa-miR9 and BCL-6 mRNAs in EBV(+) Raji and EBV-Ramous cells in mRNA levels were detected using fluorescence quantitative PCR (QRT-PCR). The two cells lines were transiently transfected with hsa-mir9-inhibitor and hsa-mir9-minicsvia Oligofectamine 2000, and the changes in BCL6 expressions was detected using QRT-PCR and Western blotting. Annexin V/PI staining was used to analyze the apoptosis and morphological changes of the transfected cells.
RESULTSThe expression of Hsa-miR9 and BCL-6 was significantly higher in EBV(+) Raji cells than EBV(-) Ramous cells (P<0.01). BCL-6 mRNA and protein expression was reduced in EBV(+) Raji cells after transfection with hsa-miR9-inhibitor but up-regulated in EBV(-) Ramous cells transfected with hsa-miR9-minics. Flow cytometry revealed a significantly decreased apoptosis rate in EBV(+) Raji cells transfected with hsa-miR9-inhibitor but an increased rate in EBV(-) Ramous cells transfected with hsa-miR9-minics, and the results were confirmed by microscopic observations.
CONCLUSIONHsa-miR9 positively regulate the expression of BCL-6 and apoptosis of EBV(+) Raji cells and EBV(-) Ramous cells.
