Changes in gene expression profiles in multiple myeloma cells after knocking-down SNF5, a core subunit of SWI/SNF complex.
- Author:
Yingying XIE
1
;
Yang XU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Chromosomal Proteins, Non-Histone; genetics; DNA-Binding Proteins; genetics; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Humans; Multiple Myeloma; genetics; pathology; RNA, Small Interfering; SMARCB1 Protein; Transcription Factors; genetics; Transcriptome
- From: Journal of Southern Medical University 2013;33(5):667-671
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in the gene expression profiles in a multiple myeloma cell line after knocking-down the expression of SNF5, a core component of the SWI/SNF chromatin remodeling complex.
METHODSThe total RNA were extracted from tetracycline-inducible SNF5 knockdown (KD) cell line derived from KM3 cells for gene expression profiling by affymetrix microarray and bioinformatic analysis.
RESULTSKnockdown of SNF5 inhibited KM3 cell proliferation. A total of 545 genes were found to be differentially expressed in the cells with SNF5 knockdown, among which 214 were up-regulated and 331 were down-regulated.
CONCLUSIONSSNF5 is essential for the growth of multiple myeloma cells and can regulate the expression of the genes associated with cell growth and apoptosis.
