Expression of epithelial-mesenchymal transition markers in tumor tissues generated by side population and non-side population of A431 cells in NOD/SCID mice.
- Author:
Lingqiao SHAO
1
;
Qianqian WANG
;
Yuanyuan GUO
;
Songmei GENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers, Tumor; metabolism; Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Tumor; Epithelial-Mesenchymal Transition; Humans; Male; Mice; Mice, Inbred NOD; Mice, SCID; Neoplastic Stem Cells; metabolism; Side-Population Cells; metabolism; Skin Neoplasms; metabolism; pathology
- From: Journal of Southern Medical University 2013;33(5):733-737
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate the side population (SP) and non-side population (NSP) cells from A431 cells and compare their difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition (EMT) markers.
METHODSA431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL, and Erbb3 in the tumor tissues were detected by immunohisto-staining.
RESULTSThe tumors generated by SP cells were larger than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3 and E-cadherin.
CONCLUSIONThe SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in tissues.
