Expression of Dickkopf-1 in human colon carcinoma cell lines.
- Author:
Qian YU
1
;
Lei YANG
;
Yanqing DING
;
Zhen ZHAO
;
Huiping LIU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line, Tumor; Colonic Neoplasms; metabolism; pathology; HCT116 Cells; HT29 Cells; Humans; Intercellular Signaling Peptides and Proteins; metabolism; Real-Time Polymerase Chain Reaction
- From: Journal of Southern Medical University 2013;33(6):923-933
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of Dickkopf-1(DKK1) in 6 different colon carcinoma cell lines.
METHODSThe expression of DKK1 protein were detected in SW480, SW620, HT29, LS174T, LOVO, and HCT116 cells lines using Western blot analysis and immunocytochemistry, and the mRNA levels of DKK1 was detected using quantitative real-time PCR (qPCR).
RESULTSThe results of qPCR showed significantly higher expressions of DKK1 mRNA in HT29 and HCT116 cells than in the other 4 cells (P<0.05). Western blotting demonstrated that DKK1 was over-expressed in HCT116 and HT29 cells compared with that in the other cell lines. All the 6 cell lines were found to have positive DKK1 expression by immunocytochemical staining.
CONCLUSIONDKK1 shows differential expression patterns among the 6 human colon carcinoma cell lines.
