Effect of folic acid in preventing aberrant methylation of fetal endometriosis susceptibility gene HOXA10.
- Author:
Mubiao LIU
1
;
Xuemei HUANG
;
Surong XU
;
Lei LI
Author Information
- Publication Type:Journal Article
- MeSH: DNA Methylation; drug effects; Endometriosis; genetics; metabolism; Female; Fetus; metabolism; Folic Acid; pharmacology; therapeutic use; Homeodomain Proteins; genetics; metabolism; Humans; Pregnancy; Promoter Regions, Genetic
- From: Journal of Southern Medical University 2013;33(6):926-929
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect aberrant methylation in the promoter region of fetal endometriosis susceptibility gene homeobox-10 (HOXA10) in women with and without folic acid supplementation and explore the effect of folic acid in optimizing intrauterine environment.
METHODSThirty-six cord blood specimens were collected between January, 2010 and December, 2012 from pregnant women with endometriosis, including 22 with folic acid treatment and 15 without. Methylation-specific polymerase chain reaction (MSP) and bisulfite salt modified sequencing (BSP) were employed to detect aberrant methylation of HOXA10 gene in these specimens.
RESULTSThe methylation rate of HOXA10 gene differed significantly between pregnant women with endometriosis taking folic acid and those who did (P<0.05).
CONCLUSIONFolic acid treatment can significantly reduce the methylation rate of fetal endometriosis susceptibility gene HOXA10.
