Desmoplakin expression silencing affects cardiac voltage-gated sodium channel Nav1.5 in HL-1 cells.
- Author:
Qianhuan ZHANG
1
;
Chunyu DENG
;
Fang RAO
;
Xiaoying LIU
;
Liping MAI
;
Jiening ZHU
;
Honghong TAN
;
Shulin WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Desmoplakins; genetics; metabolism; Gene Silencing; Mice; Mutation; Myocytes, Cardiac; metabolism; NAV1.5 Voltage-Gated Sodium Channel; metabolism
- From: Journal of Southern Medical University 2013;33(7):983-989
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of desmoplakin with the distribution and function of Nav1.5 by RNA silencing technology in HL-1 cells.
METHODSHL-1 cells with desmoplakin expression suppression by RNA silencing were examined for desmoplakin and Nav1.5 protein expressions by Western blotting, and the distribution and co-location of desmoplakin and Nav1.5 protein were detected by immunofluorescence staining. Patch-clamp recording was applied to analyze the changes in whole-cell sodium current after desmoplakin silencing.
RESULTSCompared with the untreated group and negative control group, the cells with desmoplakin silencing showed obviously reduced expressions of desmoplakin and Nav1.5 proteins. Co-localization of desmoplakin and Nav1.5 was detected at cell-cell contact in untreated and control conditions, and desmoplakin expression silencing induced a drastic redistribution of Nav1.5 with decreased peak current density (156.3∓6.2 vs 41.8∓3.1, n=6, P<0.05), a shift in voltage dependence of steady-state inactivation (-42 mV vs -61 mV, n=5, P<0.05), and prolonged time of recovery from inactivation.
CONCLUSIONDesmoplakin silencing caused redistribution of Nav1.5 protein and also changes in its electrophysiological properties in HL-1 cells.
