Expression of excision repair cross-complementation gene in drug-resistant process of carboplatin administration in tongue squamous cell cancer(Tca8113)
10.3760/cma.j.issn.1002-0098.2011.07.012
- VernacularTitle:舌鳞状细胞癌对卡铂耐药过程中切除修复交叉互补基因的表达
- Author:
Xian LI
1
;
Yong LI
;
Li-Hua QIU
;
Xi TONG
;
Qi-Ming WANG
;
Ting LI
Author Information
1. 重庆医科大学附属口腔医院
- Keywords:
Carcinoma,squamous cell;
Drug resistance,neoplasm;
Carboplatin
- From:
Chinese Journal of Stomatology
2011;46(7):437-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlations between the resistant degree of Tca8113 agaist carboplatin and the expression of excision repair cross-complementation 1(ERCC-1).MethodsTca8113 cells were exposed in the carboplatin solutions of gradually increasing concentration. Cancer cells were divided into six experimental groups and a control group. In the course of culture, the half inhibitory concentration(IC50)of each groups were detected by methyl thiazolyl tetrazolium(MTT).The expressions of ERCC-1 at mRNA and protein level were detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blotting. Results With the increase of drug resistance ,the expression of ERCC-1 increased gradually at mRNA level(control group:0.84±0.06,group 1:0.96±0.06,group 2:1.07±0.11,group 3:1.13±0.09,group 4:1.19±0.08,group 5:1.29±0.07,group 6:1.47±0.08),values between neighboring or interval groups have significant differences(P<0.05);the expression of ERCC-1 increased gradually at protein level(control group:0.69±0.05,group 1:0.86±0.04,group 2:1.01±0.04,group 3:1.24±0.09,group 4:1.44±0.14,group 5:1.56±0.19,group 6:1.88±0.22),values between neighboring or interval groups have significant differences(P<0.05). The gradually increasing RT-PCR gray values were related to the gradually increasing IC50 in all groups(r=0.91,P<0.01),and the gradually increasing western blotting gray values were also related to the gradually increasing IC50 in all groups(r=0.88,P<0.01). Conclusions The high expression of ERCC-1 may become an indicator of Tca8113 resisting carboplatin. This might provide a potential target to reverse tongue squamous cell cancer(Tca8113)that has resistance to carboplatin.
