Expression of galectin-1 in carcinogenesis of oral mucosal epithelium.
- Author:
Kui-Jie WEI
1
;
Hong-Ya PAN
;
Xiao YANG
;
Lai-ping ZHONG
;
Dong-Xia YE
;
Zhi-Yuan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Transformed; Cell Line, Tumor; Cell Transformation, Neoplastic; Epithelial Cells; cytology; metabolism; Female; Galectin 1; genetics; metabolism; Humans; Male; Middle Aged; Mouth Mucosa; metabolism; pathology; Mouth Neoplasms; metabolism; pathology; RNA, Messenger; metabolism; Up-Regulation
- From: Chinese Journal of Stomatology 2011;46(9):524-527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of galectin-1 in oral squamous cell carcinoma(OSCC) and its clinical significance.
METHODSDetection of the mRNA and protein expression of galectin-1 in the in vitro cellular carcinogenesis model of OSCC, OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction (PCR), Western blotting and immunohistochemistry, respectively.
RESULTSThe value of galectin-1 mRNA and protein level in human immortalized oral epithelia cell (HIOEC) cell was 0.071 ± 0.023, 0.118 ± 0.046, Compared with the HIOEC, galectin-1 mRNA level and protein expression were increased significantly in all the cell lines (0.141 ± 0.049, 0.504 ± 0.33) (P < 0.01). The levels of mRNA and protein expression of galectin-1 were significantly higher in the cancerous tissue (0.059 ± 0.034, 1.5 ± 0.68) than in the normal adjacent tissues (0.029 ± 0.012, 0.4 ± 0.56) (P < 0.01).
CONCLUSIONSThe expression of galectin-1 gene up-regulated in carcinogenesis process of OSCC significantly may be related to the tumorigenesis and development of OSCC, which illustrates its potential clinical application as tumor marker for early diagnosis.
