Analysis of community composition in dental plaque of elder people with root caries
10.3760/cma.j.issn.1002-0098.2011.10.005
- VernacularTitle:老年根面龋牙菌斑细菌组成的聚合酶链反应-变性梯度凝胶电泳分析
- Author:
Shan-Fen MA
1
;
Jing-Ping LIANG
;
Yun-Tao JIANG
;
Cai-Lian ZHU
Author Information
1. 上海交通大学医学院附属第九人民医院(口腔医学院)
- Keywords:
Root caries;
Dental plaque;
Bacterial floral;
Denaturing gradient gel electrophoresis
- From:
Chinese Journal of Stomatology
2011;46(10):590-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the community in dental plaque of elder people with root caries.Methods Total DNAs were extracted from the root caries dental plaques of nine elders over 60 years of age.Polymerase chaid reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microbial composition,DGGE bands were excised from the gels for sequencing and identification.Results The dominant genus in root caries dental plaque of elder people were:Acinetobacte [0.9％ (1/114) ],Actinobaculum [ 1.8％ ( 2/114 ) ],Actinomyces [ 15.8％ ( 18/114 ) ],Aggregatibacter [0.9％ (1/114) ],Capnocytophaga [ 14.0％ ( 16/114 ) ],Corynebacterium [ 0.9％ ( 1/114 ) ],Haemophilus [0.9％ ( 1/1 14 ) ],Mobiluncus [ 0.9％ ( 1/114 ) ],Naxibacter [ 0.9％ ( 1/1 14 ) ],Neisseriaceae [ 10.5％ (12/114) ],Porphyromonas [ 0.9％ ( 1/114 ) ],Prevotella [ 12.3％ ( 14/114 ) ],Selenomonas [ 6.1％(7/114 ) ],Staphylococcus [ 1.8％ ( 2/114 ) ],Oralis streptococcus [ 6.1％ ( 7/114 ) ],Mutans streptococcu [7.9％ (9/114) ],Tannerella [0.9％ ( 1/114)],Treponema [ 1.8％ (2/114)],Veillonella [ 10.5％(12/114) ] and two uncultured unknown genus [ 1.8％ (2/114 )].Uncultred genotypes accounted for 19.30％ of the total.Gram-positive bacteria genotype accounted for 31.6％ (36/114),and Gram-negative bacteria genotype accounted for 66.7％ (76/114).Conclusions There were many bacteria genotypes in root caries dental plaque in the elderly,which were widely distributed.Gram-negative bacteria accounted for the majority.Genotype-specific pathogenic bacteria were not found.
