Construction of tissue engineering bone with bone marrow stromal cell sheets

VernacularTitle:应用骨髓基质细胞片层构建组织工程骨的实验研究
Author: Ling-Xue BU ¹ ; Yan-Hui WANG ; Ning-Yi LI ; Zheng-Hua GAO ; Xin CHEN ; Heng JING
Author Information

1. 青岛大学医学院附属医院
Keywords: Bone marrow; Bone marrow cells; Tissue-engineered bone
From: Chinese Journal of Stomatology 2011;46(12):747-750
CountryChina
Language:Chinese
Abstract: Objective To construct tissue engineering bone with bone marrow stromal cell(BMSC)sheets of dogs.Methods BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis.Allogeneic dogs decalcification bone matrixes(DBM) were prepared.Sixteen dogs were divided into 4 groups.The MSC cell sheets-rhBMP2-BMSC-DBM were implanted under the left latissimus dorsi myofascial as the experimental side; while the thBMP-2-BMSC-DBM were implanted in the right side as the control.Ectopic bone formation in vivo was evaluated by histological examination 4,8,12,16 weeks after operation.Results The osteogenesis in the experimental group was better than that in the control group.New bone area in the experimental side was larger than that in the control group,and the difference was significant ( P ＜ 0.05 ).After 16 weeks,lamellar bone was connected into a film in the experimental group.Haversian system and red bone marrow could be seen.Conclusions BMSC cell sheets could promote the bone formation of functional tissueengineered bone.