Determination of 2, 4-dichlorophenoxyacetic acid in air of workplace by high-performance liquid chromatography.
- Author:
Yanan WEN
1
;
Zhaohui FU
;
Jianning XU
;
Shichuan TANG
;
Quankai WANG
;
Huanhuan LI
;
Guangyun XIE
;
Yuling ZHU
;
Yiting GU
;
Feng TAN
2
Author Information
- Publication Type:Journal Article
- MeSH: 2,4-Dichlorophenoxyacetic Acid; analysis; Air; analysis; Air Pollutants, Occupational; analysis; Chromatography, High Pressure Liquid; methods; Workplace
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(6):458-459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for determination of 2, 4-dichlorophenoxyacetic acid (2, 4-D) in the air of workplace by high-performance liquid chromatography.
METHODS2, 4-D was collected by ultrafine glass filters, desorbed by methanol, separated by a C18 column, and detected by a UV detector. Identification and quantification of 2, 4-D were performed by retention time and peak areas, respectively.
RESULTSThe linear range of the test was 2∼200 µg/ml; the elution efficiency was 94.6%- 95.9%; the limit of detection (S/N = 3) was 0.034 µg/ml (injection volume of 20 µl eluant); the lower limit of quantification (S/N = 10) was 0.11 µg/ml; the minimum detectable concentration was 0.011 mg/m(3); the minimum quantifiable concentration was 0.037 mg/m(3) (with sampled air volume of 45 L).
CONCLUSIONThis method is convenient and simple in sample collection and preparation, and satisfies all methodological requirements. Therefore, this method is useful for the determination of 2, 4-D in the air of workplace.