The Characterization of T Cell - Derived Neutrophil Survival Factor %.
- Author:
Erk HER
;
Sung Tae LEE
- Publication Type:Original Article
- Keywords:
Neutropil viability;
GM-CSF;
IL-8
- MeSH:
Adult;
Biological Assay;
Colony-Stimulating Factors;
Culture Media, Conditioned;
Granulocyte-Macrophage Colony-Stimulating Factor;
Heparin;
Humans;
Interleukin-8;
Lymphocytes;
Neutrophils*;
T-Lymphocytes;
Tissue Donors
- From:Korean Journal of Immunology
1997;19(3):363-374
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It has been reported that the stimulated T lymphocytes might secret a neutrophil survival factor. Thus the goal of study was to determine which molecules are the neutrophil survival factors secreted from the phytohaemagglutinin (PHA)-stimulated T lymphocytes. Human peripheral blood T lymphocytes and neutrophils were isolated by Ficoll-paque density sedimentation from heparinized blood of healthy adult donors. The purity of T lymphocytes and neutrophils were more than 90% and 95%, respectively. The maximal effective condition for the neutrophil viability-sustaining activity was 1 ug/ml af PHA in 12 hours incubation with T lymphocytes. The effect of PHA-stimulated T lymphocyte conditioned medium (TCM) on the neutrophils were used for the comparison with PHA-nonstimulated TCM or enriched medium alone. Neutrophil viability-sustaining activity with PHA-stimulated TCM for 24 hours incubation was significantly higher than other groups (85+/-11 vs 43+/-5 vs 916%; p<0.01). In the analysis of the primary data, the good candidates for the neutrophil viability-sustaining factor were granulocyte/monocyte colony stimulating factor (GM-CSF) and interleukin-8 (IL-8). They were used in the bioassay and antibody neutralization of cytokine activity. ...continue...
