Research on relationship of survivin gene expression with malignant proliferation and apoptosis of brain glioma.
- Author:
Hai-ning ZHEN
1
;
Xiang ZHANG
;
Pei-zhen HU
;
Luo-an FU
;
Tong-tao YANG
;
Fu-cheng MA
;
Xi-ling WANG
;
Jing-wen LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Apoptosis; Brain Neoplasms; genetics; metabolism; pathology; Cell Proliferation; Child; Child, Preschool; Female; Glioma; genetics; metabolism; pathology; Humans; Immunohistochemistry; In Situ Nick-End Labeling; Inhibitor of Apoptosis Proteins; Male; Microtubule-Associated Proteins; biosynthesis; genetics; Middle Aged; Neoplasm Proteins; biosynthesis; genetics; Proliferating Cell Nuclear Antigen; biosynthesis
- From: Chinese Journal of Surgery 2005;43(13):885-888
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression level of inhibitor of apoptosis protein survivin gene in human brain glioma and its role in malignant proliferation and antiapoptosis of brain glioma.
METHODSEighty-three cases of brain glioma specimen was chosen, protein expression of survivin and proliferating cell nuclear antigen (PCNA) was investigated by immunohistochemistry streptavidin-biotin complex (SABC) method, the immunoreactivity score (IRS) of survivin and the proliferative index (PI) were counted. Apoptotic cells were screened by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) method, and the apoptotic index (AI) of brain glioma was calculated.
RESULTSThe survivin IRS, PI and AI of brain glioma were 3.8 +/- 3.9, (28.4 +/- 19.5)% and (1.0 +/- 0.8)% respectively, and all of them were elevated with the increase of pathological grade of brain glioma (P < 0.01 for all). PI in survivin positive group was significantly higher than that in survivin negative group (P < 0.01), and PI was positively correlated with survivin IRS (r = 0.740, P < 0.01). There was no significant difference between AI in survivin positive group and that in survivin negative group (P > 0.05), however, AI was negatively correlated with survivin IRS (r = -0.307, P < 0.01).
CONCLUSIONSSurvivin is overexpressed in brain glioma, and which may play important roles in malignant proliferation and antiapoptosis of brain glioma.
