Radiosensitivity on colorectal neoplasms by recombinant adenoviral-mediated wild-type p53 gene.
- Author:
Bin-bin CUI
1
;
Ming LIU
;
Peng ZHAO
;
Jia-hong ZHAO
;
Xin-shu DONG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Colorectal Neoplasms; genetics; pathology; radiotherapy; Genes, p53; radiation effects; Genetic Vectors; Humans; Mutation; Radiation Tolerance; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Surgery 2005;43(15):1002-1005
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the radiosensitization on the cells of colorectal cancer transfected with recombinant adenovirus vector-mediated wild-type p53.
METHODSSW480 cells transfected by wild-type p53 were treated with 4 Gy and 6 Gy radiation. The expression of recombinant adenovirus vector-mediated wild-type p53 gene was detected by Western blotting. The inhibition rate of SW480 cells was examined by MTT, apoptotic rate by TdT-mediated dUTP nick end labeling (TUNEL) and proliferating cell nuclear antigen (PCNA) by immunohistochemical method.
RESULTSSW480 cells transfected by wild-type p53 were inhibited significantly by 4 Gy and 6 Gy radiation. The level of apoptosis increased and the expression of PCNA decreased.
CONCLUSIONCells of colorectal carcinoma transfected with wild-type p53 increases their radiation sensitivity.
