The dynamic effects of allogenic transplantations with the bladder acellular matrix grafts of rabbits.
- Author:
Yong-quan WANG
1
;
Xiu-guo GAN
;
Rui-hua AN
;
Cheng ZHANG
;
Yan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Extracellular Matrix; transplantation; Rabbits; Regeneration; Tissue Engineering; Transplantation, Homologous; Urinary Bladder; cytology; physiology; transplantation
- From: Chinese Journal of Surgery 2005;43(18):1219-1222
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the dynamic effects of allogenic transplantations with the bladder acellular matrix grafts (BAMG) of rabbits.
METHODSHemi-cystectomies were performed in 25 rabbits, and the defects were repaired with BAMG about half bladder size. The rabbits underwent postoperative assessment of bladder function at 8 weeks, including cystometry, vesical volume, vesical compliance and cystography. The allografts were observed by light microscope and electron microscope at 1, 2, 4, 8, 12, 16 weeks after surgery.
RESULTSMacroscopic observation revealed that BAMG regenerated gradually. All urodynamic results of 8 weeks after surgery were not different statistically as compared with these of preoperation (P > 0.05). Cystography revealed that the morphous of bladder was recovered. Epithelialization and neovascularity occurred accompanied by infiltration of inflammatory cell at 1 week. Smooth muscle cell and stratified epithelium regenerated 2 weeks after grafting. Neural elements formed around smooth muscle bundles as early as 4 weeks. Each component regenerated on the frame of BAMG sequentially. After 16 weeks, it was difficult to delineate the junction between the host bladder and BAMG by histology.
CONCLUSIONAfter allogenic transplantation with rabbits' BAMG, the constitution and function of the allografts regenerate completely and gradually on the frame of BAMG.
