The role of tissue factor in the invasion and metastasis of colorectal carcinoma cells.
- Author:
Yuan-lian WAN
1
;
Long RONG
;
Yu-cun LIU
;
Hong-wei YAO
;
Xin WANG
;
Tao WU
;
Yi-sheng PAN
;
Jing ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Colorectal Neoplasms; genetics; pathology; DNA, Antisense; genetics; DNA, Complementary; genetics; Humans; Liver Neoplasms; secondary; Lung Neoplasms; secondary; Male; Mice; Mice, Nude; Neoplasm Invasiveness; Neoplastic Cells, Circulating; pathology; Thromboplastin; biosynthesis; genetics; physiology; Transfection
- From: Chinese Journal of Surgery 2005;43(19):1265-1267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of tissue factor (TF) in the invasion and hematogenous metastasis of human colorectal carcinoma cells.
METHODSThe eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 and LoVo cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western Blot. Then the transfected and un-transfected tumor cells were implanted into nude mice (Balb/c Nu/Nu) to produce primary tumor, lung metastasis and liver metastasis respectively.
RESULTSHT-29 and LoVo cells with sense-TFcDNA transfection showed increased TF expression compared with the cells without transfection, but the cells with antisense-TFcDNA transfection got the contrary change. The primary tumor growth and invasive range, lung metastasis and live metastasis all increased in sense transfectants but reduced in antisense transfectants.
CONCLUSIONSTF can increase the invasion and hematogenous metastatic ability of human colorectal carcinoma cells.
