The study of the effect of antisense oligonucleotide specific to K-ras point mutation on human pancreatic carcinoma cell PC-2.
- Author:
Yong-xiang WANG
1
;
Liang GAO
;
Zong-zheng JI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; drug effects; Genes, ras; genetics; Humans; Oligonucleotides, Antisense; genetics; pharmacology; Pancreatic Neoplasms; genetics; pathology; Point Mutation; genetics; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Surgery 2005;43(21):1387-1390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of antisense oligonucleotide specific to K-ras point mutation on human pancreatic carcinoma cell PC-2 in vitro.
METHODSHuman pancreatic carcinoma cell PC-2 was transducted with antisense oligonucleotide specific to K-ras point mutation by liposome; the expression of target gene was studied with immunohistochemistry and in situ hybridization. The effect on cell proliferation was studied by artificial count, MTT and mass test.
RESULTSThe expression degree of ras protein and K-ras mRNA transducted with antisense oligonucleotide decreased apparently compared with control group and sense oligonucleotide group 48 h after tansduction. The inhibitory effect on cell proliferation was confirmed by artificial count, MTT and mass test.
CONCLUSIONSAntisense oligonucleotide specific to K-ras point mutation has an apparent inhibitory effect on target gene expression and cell proliferation of human pancreatic carcinoma cell in vitro.
