Arresten expressed in vivo suppresses the growth of SGC-7901 tumor xenografts in nude mice.
- Author:
Can-rong LU
1
;
Lin CHEN
;
Chun-qing DOU
;
Wen-bin CHEN
;
Chen LIN
Author Information
- Publication Type:Journal Article
- MeSH: Angiogenesis Inhibitors; biosynthesis; genetics; Animals; Cell Proliferation; Genetic Therapy; Humans; Mice; Mice, Nude; Neoplasm Transplantation; Stomach Neoplasms; pathology; therapy; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Surgery 2005;43(21):1391-1394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effect of arresten on the growth of SGC-7901 tumor xenograft nude mice model with the localized expression of arresten.
METHODSThe secretable eukaryotic expression vector pcDNA3.1 (+)-ss-arresten was constructed by molecular clone strategy, and then was transfected into human gastric cancer cell line SGC-7901 using liposome. The Western blot method was used to examine whether the protein was secreted into cell medium, and the biological behaviors of genetically modified SGC-7901 cell clone was further investigated with MTT and flow cytometry analysis system (FCAS). At last, the SGC-7901 cells expressing arresten were implanted subcutaneously into nude mice, and the weights of tumor xenografts were recorded and analyzed.
RESULTSThe eukaryotic expression vector containing secretable arresten cDNA was constructed successfully. The SGC-7901 cell line with the character of expression of arresten was obtained. The growth of arresten cDNA genetic-modified SGC-7901 tumor xenograft was suppressed.
CONCLUSIONSThe anti-tumor effect of arresten in the SGC-7901 xenograft is by inhibition of the proliferation of vascular endothelial cell of the tumor.
