Effect of endothelin-1 and its antagonists on the expression of endothelin receptors mRNA in HSC-T6 cells.
- Author:
Jun ZHANG
1
;
Zhong-tao ZHANG
;
Yu WANG
;
Ping WANG
;
Jian-she LI
;
Yan-zhong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Endothelin-1; pharmacology; Gene Expression Regulation; drug effects; Hepatocytes; drug effects; metabolism; Oligopeptides; pharmacology; Peptides, Cyclic; pharmacology; Piperidines; pharmacology; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Receptor, Endothelin A; biosynthesis; drug effects; genetics; Receptor, Endothelin B; biosynthesis; drug effects; genetics
- From: Chinese Journal of Surgery 2005;43(21):1395-1397
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of endothelin-1 (ET-1) and its antagonists on the expression of endothelin and its receptors mRNA in HSC-T6 cells.
METHODSCultured HSC-T6 cells were randomly divided into 7 groups: Sham control group, ET-1 group (10 nmol/L ET-1), BQ-123 group [1 micromol/L BQ-123, a selective endothelin receptor A (ETRA) antagonist], BQ-788 group [1 micromol/L BQ-788, a selective endothelin receptor B (ETRB) antagonist], ET-1 + BQ123 group (10 nmol/L ET-1 + 1 micromol/L BQ-123), ET-1 + BQ-788 group (10 nmol/L ET-1 + 1 micromol/L BQ-788) and ET-1 + BQ-788 group (10 nmol/L ET-1 + 1 micromol/L BQ-123 + 1 micromol/L BQ-788). The expression of endothelin receptor mRNA of HSC-T6 cells was determined by reverse-transcription polymerase chain reaction (RT-PCR).
RESULTSThe expression of ETRA mRNA in ET-1 + BQ123 + BQ788 and ET-1 + BQ788 group was significantly lower than ET-1 group (0.329 +/- 0.044 and 0.292 +/- 0.023 vs. 0.440 +/- 0.030 P < 0.05). Compared with ET-1 group, the expression of ETRB mRNA in ET-1 + BQ788 group was down regulated obviously (0.499 +/- 0.136 vs. 0.153 +/- 0.071, P < 0.05). There was no significant difference in ET-1 + BQ123 group and ET-1 + BQ123 + BQ788 group when compared with ET-1 group (0.499 +/- 0.136 vs. 0.496 +/- 0.103 and 0.299 +/- 0.129, P > 0.05).
CONCLUSIONSET-1 has no obvious effect on the expression of ETRA mRNA in HSC-T6. ET-1 may up-regulate the expression of ETRB mRNA. Act on ETRA receptor, ET-1 can inhibit the expression of ETRB mRNA.
