Chinese medicine Extractum trametes robiniophila murr augment tumor necrosis factor related apoptosis-inducing ligand induced apoptosis in human hepatic cancer cell lines.
- Author:
Xiao-ping CHEN
1
;
Song-qing HE
;
Xu ZHAO
;
Zhi-yong HUANG
;
Chang-hai LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Carcinoma, Hepatocellular; metabolism; pathology; Caspases; metabolism; Cell Line, Tumor; Drug Resistance, Neoplasm; drug effects; Drug Synergism; Drugs, Chinese Herbal; pharmacology; Humans; Liver Neoplasms; metabolism; pathology; Receptors, TNF-Related Apoptosis-Inducing Ligand; metabolism; TNF-Related Apoptosis-Inducing Ligand; pharmacology
- From: Chinese Journal of Surgery 2005;43(23):1524-1527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) combined with Chinese medicine, Extractum trametes robiniophila murr in human hepatic cancer cells.
METHODSHepG2 cell line resistant to adriamycin (HepG2/ADR) was induced step by step. The effects of TRAIL (100 ng/L) combined with Extractum trametes robiniophila murr (1.0 g/L) promoting apoptosis in HepG2 or HepG2/ADM were analyzed. The proliferation was observed by MTT assay and the apoptosis of cells was also observed by flow cytometry.
RESULTSHepG2/ADM was confirmed resisting to ADM. The treatment of TRAIL (100 ng/L) combined with Extractum trametes robiniophila murr (1.0 g/L) showed significant inhibitory effects on the growth of both HepG2 and HepG2/ADM, and the percentage of apoptosis was increased compared with other groups within 24 to 72 h.
CONCLUSIONSExtractum trametes robiniophila murr dramatically augmented the sensitivity of both HepG2 and HepG2/ADM to TRAIL, but only has slightly killing effects on L02. TRAIL combined with Chinese medicine treatment could be a safe and attractive strategy to drug-resistant/TRAIL-resistant tumor cells.
