Expression of nestin and neurogenin 3 in the human fetal pancreas.
- Author:
Zong-mei ZHENG
1
;
Dong-ming CHEN
;
Ling-song LI
;
Jian-ning LI
;
Li SHEN
;
Ai-li LU
;
Shu-ling WANG
;
Wei-han BAO
Author Information
- Publication Type:Journal Article
- MeSH: Basic Helix-Loop-Helix Transcription Factors; biosynthesis; genetics; Fluoroimmunoassay; Humans; In Vitro Techniques; Intermediate Filament Proteins; biosynthesis; genetics; Microscopy, Fluorescence; Nerve Tissue Proteins; biosynthesis; genetics; Nestin; Pancreas; cytology; embryology; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Surgery 2005;43(23):1537-1540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the expression of nestin and neurogenin 3 (Ngn3), the markers of pancreatic stem cells, in the human fetal pancreas.
METHODSThe human fetal pancreas tissue of 12 and 14 weeks were examined for the expression of nestin and Ngn3 using the techniques of immunofluorescence dye and RT-PCR.
RESULTSBoth nestin and Ngn3 expressed widely in 12 and 14 weeks before in human fetal pancreatic tissue. In these positive cells there was no co-expressing insulin or glucagon. There were nestin and Ngn3 co-expressing cells in ducts but not in the islets. The results of RT-PCR also indicated the expression of nestin and Ngn3.
CONCLUSIONSThere was no expression of the markers of mature endocrine cells in the nestin and Ngn3 positive cells, and they were the marks of no-differentiation cells in the human fetal pancreatic tissue.
