Effect of interleukin-8 in cell invasion and proliferation of human breast cancer.
- Author:
Ying LIN
1
;
Shen-ming WANG
;
Wei-ming LÜ
;
Ruo-pan HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; metabolism; pathology; Cell Proliferation; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunohistochemistry; Interleukin-8; biosynthesis; physiology; Neoplasm Invasiveness; Neoplasm Metastasis; Protein Array Analysis; Receptors, Estrogen; metabolism; Tumor Cells, Cultured; Tumor Suppressor Protein p53; metabolism
- From: Chinese Journal of Surgery 2005;43(23):1541-1544
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the effect of interleukin-8 in cell progression and invasion of human breast cancer.
METHODSHuman cytokine antibody arrays were applied to screen a panel of cytokine expression from 11 human breast cancer cell lines, and the mechanism of identified key factors involved in breast cancer progression was studied.
RESULTSProfiling of cytokine expression showed the expression of interleukin-8 was related to estrogen receptor status, metastasis and vimentin status in the 11 human breast cancer cell lines. Elevated expression of interleukin-8 in breast cancer cells had positive correlation with breast cancer invasion. Neutralization of antibody against interleukin-8 specifically blocked interleukin-8-mediated cell invasion. However, anti-interleukin-8 antibody did not influence the proliferation of breast cancer cells.
CONCLUSIONInterleukin-8 may be the key factor involved in human breast cancer progression and invasion, and play an important role in cell invasion of breast cancer.
