Diagnostic significance of immunophenotyping and detection of gene rearrangement in subcutaneous panniculitis-like T-cell lymphoma.
- Author:
Chuan WAN
1
;
Chen XU
;
Lin WANG
;
Gan-di LI
;
Wei JIANG
;
Wen-yan ZHANG
;
Yuan TANG
;
Bei KAN
;
Wei-ping LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Antigens, CD20; immunology; CD56 Antigen; analysis; immunology; Child; Female; Gene Rearrangement; genetics; Humans; Immunophenotyping; methods; In Situ Hybridization; Lymphoma, T-Cell; classification; diagnosis; genetics; immunology; Lymphoma, T-Cell, Cutaneous; classification; diagnosis; genetics; immunology; Male; Middle Aged; Panniculitis; RNA, Viral; immunology; isolation & purification; Skin Neoplasms; immunology; Subcutaneous Tissue; Young Adult
- From: Chinese Journal of Pathology 2008;37(6):390-394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the diagnostic implication of immunophenotyping and gene rearrangement in subcutaneous panniculitis-like T-cell lymphoma (SPTL).
METHODSAccording to the selection criteria of 2005 WHO-EORTC classification for cutaneous lymphomas, 20 SPTL patients were enrolled in this study. A 10-antibody panel was used for immunophenotyping and in addition, polymerase chain reaction for TCR gamma and IgH gene rearrangement and in situ hybridization for EBER1/2 were also employed.
RESULTSThere were 9 males and 11 female with a mean age of 29.5 years. Immunophenotypic study showed that all the patients expressed one to three T-cell associated antigens (CD2, CD3 or CD45RO), 18 patients were positive for beta F1, 18 for CD8, 20 for TIA-1 and 16 for granzyme B. None of the patients expressed CD4, CD20 and CD56. TCR gamma gene rearrangement was found in 16 of 20 cases (80.0%) and none for IgH gene rearrangement. The positive rate of EBER1/2 was 25.0% (5/20).
CONCLUSIONSSince the majority of SPTL patients show clonal TCR gene rearrangements, correlations among clinical presentation, histological features, immunophenotype and gene rearrangement data are considered important in confirming a diagnosis of SPTL.
