Differential expression of Notch1 and Notch2 in astrocytoma and medulloblastoma.
- Author:
Peng XU
1
;
Pei-Yu PU
;
Chun-Sheng KANG
;
Zhi-Fan JIA
;
Xuan ZHOU
;
Guang-Xiu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Astrocytoma; metabolism; Biomarkers, Tumor; metabolism; Brain; metabolism; Brain Neoplasms; metabolism; Child; Child, Preschool; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Medulloblastoma; metabolism; Middle Aged; Receptor, Notch1; metabolism; physiology; Receptor, Notch2; metabolism; physiology; Young Adult
- From: Chinese Journal of Pathology 2008;37(7):450-453
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the differential expression of Notch1 and Notch2 in human astrocytoma and medulloblastoma; and to study the role of Notch1 and Notch2 in the development of both tumors.
METHODSImmunohistochemical staining (SP method) and Western blot analysis were used to detect Notch1 and Notch2 expression in tissue arrays and freshly resected samples of normal brain tissue, astrocytoma and medulloblastoma.
RESULTSNotch1 and Notch2 were negative in normal human brain tissue. Notch1 was highly expressed (total positive rate 80.0%, 48/60) while Notch2 was not detected in grade IV astrocytomas and sporadically observed in lower grade astrocytomas (total positive rate 10.0%, 6/60). The percentage of positive tumor cells and expression level of Notch1 increased with higher histologic grade (r = 0.859, P < 0.05). On the other hand, overexpression of Notch2 was detected in medulloblastoma (9/10) in contrast with lower expression of Notch1 (2/10).
CONCLUSIONSNotch1 and Notch2 show differential expression in astrocytoma and medulloblastoma. This may be related to their different functional activities during the process of brain development.
