Proteomics study of papillary thyroid carcinoma.
- Author:
Jie SHI
1
;
Zhao-Hui LU
;
Quan-Cai CUI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Biomarkers, Tumor; analysis; metabolism; Carcinoma, Papillary; pathology; Down-Regulation; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Middle Aged; Neoplasm Proteins; analysis; metabolism; Proteomics; methods; Thyroid Gland; chemistry; pathology; Thyroid Neoplasms; pathology; Up-Regulation; Young Adult
- From: Chinese Journal of Pathology 2008;37(7):465-470
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the proteomics of papillary thyroid carcinoma (PTC), and to detect the differential expression of proteins compared to normal thyroid (NT) tissue.
METHODSProtein extracts of papillary thyroid carcinoma and normal thyroid tissues from 10 patients were separated by proteomics technique and identified using Image Master software. The selected differential proteins were sent to Proteomics Research Center of Chinese Academy of Medical Sciences for mass-spectrometry identification.
RESULTSThe basic 2-dimensional electrophoresis (2-DE) images of PTC and NT were obtained, and four potential differential expression spots were selected for mass-spectrometry identification. Among them, annexin I, peroxiredoxin I, mitochondrial aconitase were up-regulated while carbonic anhydrase I was down-regulated.
CONCLUSIONProteomics may provide a new direction to study PTC, by identifying some tumor-specific markers, leading to elucidation of the mechanism of tumorigenesis and identification of therapeutic targets.
