Preparation of hollow fiber bioreactor for culturing pig hepatocytes.
- Author:
Ying-jie WANG
1
;
Hong-ling LIU
;
Hai-tao GUO
;
Jun LIU
;
Hong-wei WEN
;
Yu-ming WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bioreactors; Biotechnology; Cells, Cultured; Female; Hepatocytes; cytology; Liver; cytology; physiology; Liver, Artificial; Male; Swine; Swine, Miniature
- From: Chinese Journal of Hepatology 2003;11(6):358-360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the method of preparing the hollow fiber bioreactor for culturing pig hepatocytes.
METHODSHepatocytes were isolated from experimental suckling minipigs by two-step perfusion with collagenase, and seeded onto hollow fiber bioreactor, then cultured with an artificial capillary cell culture system. The albumin-excretion, lidocaine-transforming rate, lactate dehydrogenase (LDH) release and the cell viability in bioreactors were examined.
RESULTSThe porcine albumin could be detected by SDS/PAGE on the 2nd, 4th, 6th day. The rates of lidocaine-transforming ranged from 89.6% to 96.1%. The release of LDH into the culture medium increased from (23.7+/-4.6) U/L to (127.8+/-17.4) U/L (F=39.582, P<0.01) during the experiments, and the viability of pig hepatocytes in hollow fiber bioreactor reduced from 95.8%+/-0.3% to 83.8%+/-4.7% (t=5.135, P<0.01).
CONCLUSIONThe hollow fiber bioreactor for culturing pig hepatocytes can be prepared by artificial capillary cell culture system, which provides a certain liver-specific function in 1 week.
