Specific immune responses in Balb/c mice induced by plasmid coexpressing hepatitis B surface antigen and granulocyte-macrophage colony stimulating factor.
- Author:
Yu-ling QING
1
;
Ning LING
;
Jia-jiang ZHAO
;
Ming-li PENG
;
Hong-mei XU
;
Hong REN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Enzyme-Linked Immunosorbent Assay; Female; Granulocyte-Macrophage Colony-Stimulating Factor; biosynthesis; genetics; immunology; Hepatitis B Surface Antigens; biosynthesis; genetics; immunology; Hepatitis B Vaccines; immunology; Interferon-gamma; biosynthesis; Interleukin-2; biosynthesis; Interleukin-4; biosynthesis; Lymphocyte Activation; Male; Mice; Mice, Inbred BALB C; Vaccines, DNA; immunology
- From: Chinese Journal of Hepatology 2003;11(8):474-476
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the improvement of specific immune responses induced by plasmid coexpressing hepatitis B surface antigen (HBsAg) and granulocyte-macrophage colony stimulating factor (GM-CSF).
METHODSAll Balb/c (H-2d) mice were immunized with pGM-CSF/S, pS/GM-CSF, pS or control plasmids. 4 weeks later, anti-HBs titer and the levels of IL-2, IL-4 and IFN-gamma in the supernatant of splenocytes were detected using enzyme- linked immunosorbent assay (ELISA), and HBsAg-specific cytotoxic T lymphocytes (CTL) activity was measured with a 51Cr release assay, using P815/S transfectants as target cells.
RESULTSThe anti-HBs antibody titers in the serum, the levels of IL-2 and IFN-gamma, and the CTL activity in pcDNA3.1-GM-CSF-S immuned mice were higher than those in PcDNA3.1-S immunized mice (F=4.176, P<0.01; F=31.188, P<0.01; F=31.796, P<0.01; F
CONCLUSIONIt will improve the specific immune responses induced by HBsAg DNA vaccine after it is binded to the gene of GM-CSF.
