Testosterone-induced spermatogenic impairment is associated with looser arrangement of spermatogenic cells in rats.
- Author:
Xian-Zhong DENG
1
;
Bo LIAO
;
Ling-Shu KONG
;
Zheng-Wei YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Male; Rats; Rats, Sprague-Dawley; Seminiferous Tubules; cytology; drug effects; Spermatogenesis; drug effects; Testis; cytology; drug effects; pathology; Testosterone; administration & dosage; adverse effects
- From: National Journal of Andrology 2011;17(6):507-510
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether testosterone-induced intra-testicular testosterone withdrawal and therefore spermatogenic impairment is associated with looser arrangement of spermatogenic cells in rats.
METHODSAdult male SD rats received intramuscular injection of testosterone undecanoate at 19 mg/(kg x 15 d) for 130 days, and then testicular tissue blocks were obtained for the preparation of methacrylate resin-embedded sections and observation of the changes in testicular histology.
RESULTSApart from such changes as impaired spermiogenesis and spermiation, apparently looser arrangement of spermatogenic cells was seen in 11.5% of the seminiferous tubule profiles, with radial cracks (empty spaces) running towards the tubule lumen being formed between lines, bundles or groups of spermatogenic cells (mainly spermatids and spermatocytes).
CONCLUSIONLooser arrangement of spermatogenic cells is one of the key histological changes resulting from intra-testicular testosterone withdrawal in rats.
