Neuregulin promotes spermatogonia proliferation in mice.
- Author:
Ying-ji JIN
1
;
Xiao-yan PAN
;
Yu-ji JIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Intracellular Signaling Peptides and Proteins; pharmacology; Male; Mice; Mice, Inbred C57BL; Neuregulin-1; pharmacology; Signal Transduction; Spermatogonia; cytology; metabolism
- From: National Journal of Andrology 2011;17(5):406-409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of neuregulin (neural regulation of protein, NRG) in the process of mouse spermatogonia proliferation.
METHODSMouse testis fragments were cultured in the medium DMEM containing purified NRG1beta or NRG3 at the concentration of 50, 100 and 200 ng/ml, respectively, followed by BrdU immunohistochemical staining and determination of the proliferation rate of spermatogonia.
RESULTSCompared with the control group, neuregulin significantly promoted the proliferation of spermatogonia (P < 0.05). The proliferation rates of spermatogonia cultured in the medium with 50, 100 and 200 ng/ml of NRG13 were 1.69, 1.55 and 1.86 times, and those with 50, 100 and 200 ng/ml of NRG3 were 1.35, 1.54 and 2.11 times that of the control.
CONCLUSIONNRG1beta and NRG3 can promote the proliferation of mouse spermatogonia, and NRG is expected to be applied in the treatment of male infertility.
