Regulatory effect of MTA1 on the anoikis of human prostate cancer cells.
- Author:
Fei-lun CUI
1
;
Dan-dan GONG
;
Yong-jing ZHOU
;
Ling ZHU
;
Yu FAN
Author Information
- Publication Type:Journal Article
- MeSH: Anoikis; genetics; Gene Expression Regulation, Neoplastic; Histone Deacetylases; genetics; Humans; Male; Prostatic Neoplasms; genetics; RNA, Small Interfering; Repressor Proteins; genetics; Transfection; Tumor Cells, Cultured
- From: National Journal of Andrology 2011;17(5):427-430
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of MTA1 small interfering RNA (siRNA) on the anchorage-independent growth and anoikis of prostate cancer cell line PC-3.
METHODSAfter transfection of human prostate cancer PC-3 cells by MTA1 siRNA, we detected the expression of the MTA1 gene by real-time PCR and Western blot, the anchorage-independent growth of the cells by clone formation in soft agar, and their anoikis by DNA fragmentation assay and flow cytometry.
RESULTSCompared with the control group, MTA1 siRNA transfection significantly decreased the mRNA and protein levels of MTA1, inhibited the anchorage-independent growth of the PC-3 cells, and induced their anoikis, all in a dose- and time-dependent manner (r = 0.935, P = 0.001; r = 0.901, P = 0.0005; r = 0.916, P = 0.0003).
CONCLUSIONMTA1 siRNA can inhibit the anchorage-independent growth of prostate cancer cells by inducing their anoikis.
