Location of Dctn1 in the mouse testis and sperm and its role in spermiogenesis.
- Author:
Bo ZHENG
1
;
Min JIANG
;
Su-Ying LI
;
Yi-Bo WU
;
Hui ZHU
;
Zuo-Min ZHOU
;
Jia-Hao SHA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dynactin Complex; Male; Mice; Mice, Inbred ICR; Microinjections; Microtubule-Associated Proteins; genetics; metabolism; RNA, Small Interfering; Rete Testis; metabolism; Seminiferous Tubules; metabolism; Sperm Count; Sperm Motility; Spermatogenesis; Spermatozoa; metabolism; Testis; metabolism
- From: National Journal of Andrology 2011;17(9):799-804
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of dynactin 1 (Dctn1) in the process of mouse spermiogenesis.
METHODSWestern blot and indirect immunofluorescence were used to analyze the expression and location of Dctn1 in the mouse testis and spermatozoa. The highest efficiency of small interference RNA (siRNA) was verified by GC2-spd cell line in vitro and in vivo studies, respectively. Dctn1 siRNA mixed with the indicator (0.4% trypan blue) was injected into the seminiferous tubules of 3-week-old ICR mice through rete testis microinjection, and negative control siRNA injected into the control testes. The normal group included 3-week-old ICR mice that did not receive any treatment. Spermatozoa were collected from the cauda epididymis 3 weeks after siRNA injection for morphological analysis.
RESULTSDctn1 was mainly localized in the tail of spermatozoa. After interference, the sperm tail abnormality in the Dctn1 siRNA group was (23.57 +/- 0.55)%, significantly higher than (12.35 +/- 2.29)% in the control (P < 0.01, n = 3), and it was (3.37 +/- 0.69)% in the normal group.
CONCLUSIONDctn1 plays an important role in mouse spermiogenesis, and mainly affects the formation of the tail of spermatozoa.
