Expression and characterization of transcutaneous immunization adjuvant LTB and LTK63.
- Author:
Si-yong CHEN
1
;
Yao YI
;
Yu GUO
;
Sheng-li BI
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; genetics; isolation & purification; metabolism; Animals; Bacterial Toxins; genetics; immunology; metabolism; Blotting, Western; CHO Cells; Cloning, Molecular; Cricetinae; Cricetulus; Electrophoresis, Polyacrylamide Gel; Enterotoxins; genetics; immunology; metabolism; Escherichia coli; genetics; metabolism; Escherichia coli Proteins; genetics; immunology; metabolism; Gene Expression; Genetic Engineering; Plasmids; genetics; Recombinant Fusion Proteins; genetics; immunology; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(1):8-11
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study a new kind of adjuvant: transcutaneous immunization adjuvant.
METHODSThe full length gene of Heat-labile enterotoxin (LT) was amplified from E. coli H10407. The B subunit protein LTB and the nontoxic A subunit protein LTKA were expressed by genetic engineering manipulation. After purification, they were identified with SDS-PAGE, GM1-ELISA and so on.
RESULTSThe LTB protein still persisted its biologic activity that conjugated specifically with GM1 ganglioside, and the LTK63 protein lost its toxin activity.
CONCLUSIONThe results showed that LTB and LTK63 may be used as promising transcutaneous immunization adjuvant.
