Isolation and identification of arboviruses in Hebei Province.
- Author:
Huan-qin WANG
1
;
Wei-bing LIU
;
Dong-rong YANG
;
Yong LIANG
;
Jun-wei WANG
;
Lian-shan ZHANG
;
Jia-wei LIU
;
San-ju TAO
;
Xin-jun LV
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arboviruses; classification; genetics; isolation & purification; Cell Line; Cluster Analysis; Culicidae; virology; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study the arboviruses carried by mosquitoes collected in Hebei Province.
METHODSSamples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR.
RESULTSTotally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus.
CONCLUSIONGetah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.
