Isolation and identification of Japanese encephalitis virus in Liaoning Province.
- Author:
Jun-wen WANG
1
;
Shi-hong FU
;
Huan-yu WANG
;
Xiao-yan MAO
;
Wei-bin LIU
;
Ying HE
;
Xiao-hong SUN
;
Zeng-lin CAI
;
Li-ping WU
;
Xi-fang ZHAO
;
Rui-hong HAN
;
Ya JING
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cercopithecus aethiops; China; Culicidae; virology; Encephalitis Virus, Japanese; classification; genetics; isolation & purification; Phylogeny; Sequence Analysis, DNA; Vero Cells; Viral Envelope Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(1):61-65
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study arboviruses carried by mosquitoes in Liaoning Province in 2002.
METHODSTotally 4927 mosquitoes were collected from Liaoning Province in July 2002. Virus strains were isolated by inoculating BHK-21, C6/36 and Vero cells. The newly isolated strains were identified by serological (ELISA and IFA) and molecular methods (Real-Time PCR and RT-PCR).
RESULTSTwo strains were isolated from mosquitoes causing cytopathogenic effect (CPE) on cells and were fatal to suckling mice. Serological tests showed that both were positive for the antibody to JEV. The PrM and E gene were cloned and sequenced. The phylogenetic analysis showed that the new isolates belonged to genotype I, JEV. Sequence analysis showed that the homology of nucleotide sequences and amino acid (AA) sequences between the two strains was 100%. Compared with the nucleotide sequences between the two strains and the standard JEV vaccine strain SA14-14-2, the difference was up to 4.11%, and the difference of AA was 0.6%.
CONCLUSIONTwo strains of JEV were isolated and identified in Liaoning province, both belonged to genotype I JEV.
