Antigenic and genetic study of influenza virus B circulated in China in 2004-2005.
- Author:
Ye ZHANG
1
;
Le-ying WEN
;
Xiang ZHAO
;
Zi LI
;
Jun-feng GUO
;
Cui-ling XU
;
Min WANG
;
Hong-jie YU
;
Wei-zhong YANG
;
Yuan-ji GUO
;
Yue-long SHU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Viral; blood; China; epidemiology; Hemagglutinin Glycoproteins, Influenza Virus; genetics; Humans; Influenza B virus; classification; genetics; immunology; Influenza, Human; epidemiology; virology; Phylogeny; RNA, Viral; genetics; isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(2):11-13
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo analyze the genetic and antigenic characteristics of hemagglutinin (HA) gene of human influenza B virus isolated from the mainland of China since 2004-2005.
METHODSThe single-way hemagglutinin inhibition (HI) tests were used to test the antigenic characteristics, and the HA1 gene was sequenced based on the antigenic results.
RESULTSThe Yamagata-like and Victoria-like viruses co-circulated in 2004-2005. For the Yamagata-like virus, the single-way HI results showed that 3.7% and 4.5% of the viruses had 4-fold greater HI titer difference compared with B/Shanghai/361/02 in 2004 and 2005, respectively. The HA1 sequence data showed that the virus had amino acid mutation, and there was one more glycosylation site at 196th site. For the Victoria-like virus, the single-way HI results showed that 8.5% and 20.6% of the viruses had 4-fold greater HI titer difference compared with B/Hong kong/330/01 in 2004 and 2005, respectively. The HA1 sequence data showed that the virus had replacement of 9 amino acids, and there was one more glycosylation site at 197th site.
CONCLUSIONThe results showed that influenza B viruses had changed antigenic and genetic characteristics compared with B/Shanghai/361/02, B/Hong kong/330/01 in 2004-2005.
