Development of methods for detection of H5N1 from human clinical specimens.
- Author:
Le-ying WEN
1
;
Hong XU
;
Yu LAN
;
Xiang ZHAO
;
Xiao-guang ZHANG
;
Da-yan WANG
;
Li-hong YAO
;
Jie DONG
;
Jia-huai ZHANG
;
Yuan-ji GUO
;
Yue-long SHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chick Embryo; DNA Primers; genetics; Humans; Influenza A Virus, H5N1 Subtype; genetics; isolation & purification; Influenza, Human; diagnosis; virology; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; methods; Sensitivity and Specificity; Viral Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(2):24-26
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.
METHODSThe typing and sub-typing primers were designed according to M and H5 and N1 gene respectively, and the RT-PCR and real-time PCR were developed using these primers.
RESULTSThe RT-PCR and real-time PCR could be used for H5N1 detection specifically, and there was no cross reaction with other influenza subtypes such as H1 and H3. The sensitivity for RT-PCR and real-time PCR was 1 TCID50 and 0.01 TCID50 respectively. Thirteen laboratory confirmed human H5N1 cases were detected from 42 suspected cases by using these methods.
CONCLUSIONThe established RT-PCR and real-time PCR methods can be used for laboratory detection of suspected human H5N1 cases.
