Antigenic and genetic study of influenza virus (H1N1) circulated in China in 2004-2005.
- Author:
Cui-ling XU
1
;
Ni-juan XIANG
;
Ye ZHANG
;
Le-ying WEN
;
Xiang ZHAO
;
Zi LI
;
Jun-feng GUO
;
Min WANG
;
Hong-jie YU
;
Wei-zhong YANG
;
Yuan-ji GUO
;
Yue-long SHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; blood; Cell Line; China; epidemiology; Humans; Influenza A Virus, H1N1 Subtype; classification; genetics; immunology; Influenza, Human; blood; epidemiology; virology; Phylogeny; RNA, Viral; genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(2):27-29
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo analyze the genetic and antigenic characteristics of hemagglutinin (HA) gene of human influenza (H1N1) virus isolated from the mainland of China since 2004 to 2005.
METHODSThe single-way hemagglutination inhibition (HI) tests were used to test the antigenic characteristics, and the HA1 gene was sequenced based on the antigenic results.
RESULTSThe single-way HI results showed that no virus isolates had 4-folds greater HI titer compared with A/Shanghai/1/1999 (H1N1) in 2004, but there was 6.3% virus had 4-fold greater difference in 2005. The HA1 sequence data showed that the H1N1 virus had the following amino acid mutations such as 54 K > R, 90 T > K, 101 Y > H, 149 R > K, 169 V > A, 190 D > N, 212 R > K, 219 K > R, 245 W > R, 246 Y > F, 258 T > N, 318 V > A and the 54 and 190 amino acids located in antigenic group of HA1.
CONCLUSIONThe H1N1 virus was changing in antigenic and genetic characteristics.
