Immune responses in rhesus induced by recombinant adenovirus Ad-LMP2.
- Author:
Zhan WANG
1
;
Ling ZHOU
;
Xiao-bing WU
;
Mi-jia LU
;
Yao-xian XUAN
;
Jian-min ZUO
;
Feng LI
;
Qi WANG
;
Shu-qing YE
;
Yi ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Antibodies, Viral; blood; Antibody Formation; immunology; Female; Immunization; methods; Macaca mulatta; Male; Recombinant Fusion Proteins; genetics; immunology; T-Lymphocytes, Cytotoxic; immunology; Vaccines, DNA; genetics; immunology; Viral Matrix Proteins; genetics; immunology; Viral Vaccines; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(2):63-65
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo observe the LMP2 specific cellular and humoral immune responses after immunization with recombinant adenovirus Ad-LMP2 in rhesus.
METHODSThe rhesuses were immunized with Ad-LMP2 through intra muscular injection in three groups, high dosage (4.5 x 10(11) VP/kg), medium dosage (1.5 x 10(11) VP/kg) and low dosage (0.5 x 10(11) VP/kg) groups. They were totally immunized six times at intervals of 5 days. The specific cellular immune responses were tested during the 7th week by ELISPOT after immunization. And the titers of anti-LMP2 antibody were tested by EIA throughout the period of immunization.
RESULTSLMP2 induced specific cellular and humoral immune responses in all three dosage group. The potency of immune responses was related with the dosage of immunization. Higher dosage elicited more potent immune response. Both the neutralizing antibody to adenovirus and anti-LMP2 antibody could be detected from 2 weeks after immunization. They would reach the peak during 3-4 weeks after immunization, then declined during the 7th week after immunization.
CONCLUSIONThe recombinant adenovirus LMP2 could induce specific cellular and humoral responses in rhesus after immunization.
