Identification and typing of human enteroviruses using an RT-PCR assay.
- Author:
Wen-jun HOU
1
;
Jian-yang YANG
;
Yan HU
;
Ying SUN
;
Pan-yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: DNA Primers; Enterovirus; classification; genetics; Enterovirus Infections; diagnosis; Humans; RNA, Viral; genetics; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; methods; Sensitivity and Specificity; Serotyping; methods
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(2):69-71
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo establish a molecular detection and typing assay for identification and typing of human enteroviruses (HEV) which is suitable for clinical detection and epidemiologic research.
METHODSUsing both primers specific for HEV genus and HEV typing primers and reverse transcription polymerase chain reaction (RT-PCR) the authors detected preliminarily HEV by agarose gel electrophoresis and then identified serotype through nucleotide sequence analysis of RT-PCR amplicons. The monospecific antisera neutralization was applied to validate the typing results.
RESULTSThe serotype of 18 suspicious HEV samples was identified: 4 Coxsackievirus type A24 (CVA24), 3 CVB3, 1 CVB2, 1 CVA9, 1 CVA15, 1 Echovirus type 3 (E3), 1 E6, 1 E9, 1 E11, 1 E14, 1 E33 and 1 Rhinovirus type 9. The result was validated by monospecitive antisera neutralization.
CONCLUSIONThis RT-PCR assay for HEV detection and typing may be suitable for clinical detection and epidemic research since this method is sensitive and specific for direct identification and typing of HEV.
